lncap cell  (ATCC)

Journal: ChemistryOpen

Article Title: Phytochemical Profiling of the Halophyte Artemisia fukudo Makino and Its In Vitro Effects on Androgen‐Related Pathways Associated With Benign Prostatic Hyperplasia and Alopecia

doi: 10.1002/open.202500481

Figure Lengend Snippet: The effects of 90% MeOH fraction of A. fukudo (AFE) on the expressions of androgen receptor (AR), prostate‐specific antigen (PSA), and 5‐alpha reductase type 2 (5αR2) in LNCaP cells activated with TP (testosterone propionate). Cells were treated with TP (0.5 μM) and then treated with AFE (6.25, 12.5, and 25 μg/mL) or finasteride (10 μM) for 72 h. The expression levels of AR, 5αR2, and PSA in cells were analyzed by Western blotting. Band intensities were quantified by densitometry, normalized to α‐tubulin, and expressed relative to the TP‐treated group (set to 1). Data are presented as the mean ± SD (error bars) from three independent experiments ( n = 3). Statistical analysis was performed by one‐way ANOVA followed by Duncan's multiple range test; * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to TP‐treated cells. Fina, finasteride; NC, nontreated control; TP, testosterone propionate.

Article Snippet: RWPE‐1 cell (human prostatic epithelial cell line; RRID: CVCL‐3791) and LNCaP cell (human prostate adenocarcinoma cell line; RRID: CVCL‐0395) were purchased from American Type Culture Collection (ATCC, Manassas, VA, United States; CRL‐11609D and CRL‐1740).

Techniques: Expressing, Western Blot, Control

Journal: Journal of Medicinal Chemistry

Article Title: Zwitterionic Modification of PSMA Ligands Reduces Off-Target Binding and Tissue Retention

doi: 10.1021/acs.jmedchem.5c03849

Figure Lengend Snippet: Widefield fluorescence microscopy images of PC3-PIP, LNCaP and PC3 flu cells after incubation with Cy5-conjugates 8 , 9 and 10 (2.5 μ m or 10 μ m , 37 °C, 2 h) with or without blocking (100-fold excess 2-PMPA). Nuclei were stained with DAPI (cyan). The Cy5 signal is shown in magenta. λ exc (DAPI) = 395 nm; λ exc (Cy5) = 640 nm. Scale bar = 50 μm.

Article Snippet: The PSMA-positive cell line LNCaP was obtained from DSMZ (Deutsche Sammlung von Mikroorganismen and Zelllinien #ACC 256).

Techniques: Fluorescence, Microscopy, Incubation, Blocking Assay, Staining

Journal: Journal of Medicinal Chemistry

Article Title: Zwitterionic Modification of PSMA Ligands Reduces Off-Target Binding and Tissue Retention

doi: 10.1021/acs.jmedchem.5c03849

Figure Lengend Snippet: Representative confocal microscopy images of (A) PC3-PIP and LNCaP cells after incubation with Cy5 conjugates 8 , 9 and 10 (10 μ m , 2 h, 37 °C) and (B) ZW800−617 13 (10 μ m , 2 h, 37 °C). Nuclei were stained with DAPI (cyan). The Cy5 signal is shown in magenta and the ZW800 signal in yellow. Images are shown as maximum intensity projections of z-stacks acquired with a 63x oil immersion objective. λ exc (DAPI) = 405 nm; λ exc (Cy5) = 653 nm, λ exc (ZW800) = 770 nm. Scale bar = 20 μm.

Article Snippet: The PSMA-positive cell line LNCaP was obtained from DSMZ (Deutsche Sammlung von Mikroorganismen and Zelllinien #ACC 256).

Techniques: Confocal Microscopy, Incubation, Staining

Journal: Journal of Medicinal Chemistry

Article Title: Zwitterionic Modification of PSMA Ligands Reduces Off-Target Binding and Tissue Retention

doi: 10.1021/acs.jmedchem.5c03849

Figure Lengend Snippet: Full body cryo-fluorescence tomography images (maximum intensity projection) of PC3-PIP and PC3 flu tumor-bearing xenograft mice obtained 4 h p.i. of 20 nmol dose of Cy5 conjugates 8 , 9 , 10 and ZW800 conjugate 13 . Selected organs are marked with the following abbreviations: PC3-PIP = PSMA-positive tumor xenograft; PC3 flu = PSMA-negative tumor xenograft; LG = lacrimal glands; Bl = bladder; GI = gastrointestinal tract, K i = kidney. Images were captured at 45 μm/pixel resolution with the following set ups: Cy5 channel = 640 nm excitation laser and 680/13 nm emission filter; ZW800 channel = 780 nm excitation laser and 840/70 nm emission filter.

Article Snippet: The PSMA-positive cell line LNCaP was obtained from DSMZ (Deutsche Sammlung von Mikroorganismen and Zelllinien #ACC 256).

Techniques: Fluorescence, Tomography

Journal: Oncology Letters

Article Title: SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth

doi: 10.3892/ol.2025.15404

Figure Lengend Snippet: SENP5 acts as a downstream target of AR and contributes to PCa growth. (A and B) Survival analysis of patients with PCa stratified according high and low SENP5 expression using the prostate adenocarcinoma dataset from The Cancer Genome Atlas. (A) Overall survival; (B) Disease-free survival. (C and D) Immunofluorescence analysis of the expression of SENP5 in PCa tissues. (C) Representative images (including low, intermediate and high expression) from the immunofluorescence analysis for SENP5 in primary PCa tissues and corresponding adjacent normal prostate tissues. (D) Quantification of the staining observed in 30 PCa cases. (E) LNCaP and LNCaP-AI cells were transfected with siScr or siSENP5 for 48 h; western blots were probed for levels of SENP5 and GAPDH was used as a loading control. (F) Cell viability was determined using a Cell Counting Kit-8 assay. (G) LNCaP and LNCaP-AI cells were treated with MDV3100 (25 µM), DHT (10 nM) or vehicle alone for 48 h. The mRNA expression level of SENP5 was measured using quantitative PCR. (H) LNCaP cells were transfected with siAR or treated with MDV3100 (25 µM) for 48 h; then total protein cell lysates were prepared and immunoblot analysis was performed using anti-SENP5 and anti- GAPDH antibodies. (I) Chromatin immunoprecipitation-quantitative PCR showing the occupancy of AR on the human SENP5 ARE region in LNCaP cells with indicated treatment. All results are representative of three experiments and expressed as the mean ± S.D. *P<0.05. AR, androgen receptor; PCa, prostate cancer; si-, small interfering; DHT, dihydrotestosterone.

Article Snippet: LNCaP cells (cat. no. CRL-1740) were purchased from the American Type Culture Collection, cells were cultured in RPMI-1640 media (cat. no. PYG0006; Wuhan Boster Biological Technology, Ltd.) supplemented with 10% fetal bovine serum (FBS) in 37°C and 5% CO 2 atmosphere.

Techniques: Expressing, Immunofluorescence, Staining, Transfection, Western Blot, Control, Cell Counting, Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation

Journal: Molecules

Article Title: Preclinical Evaluation of a Radiolabeled Anti-PSMA Dimeric Aptamer in a Murine Model of Human Prostate Cancer

doi: 10.3390/molecules31030493

Figure Lengend Snippet: Retention of [ 89 Zr]Zr-A11 and [ 89 Zr]Zr-A12 in PSMA + LNCAP tumors (●; black symbol and line) and PSMA − PC-3 tumors ( ● ; red symbol and line). Tumor-bearing mice ( n = 4 or 5 per group) were injected with either [ 89 Zr]Zr-A12 or [ 89 Zr]Zr-A11 via the tail vein. When comparing the radioactivity retention at 1 h, 4 h and 24 h, animals receiving [ 89 Zr]Zr-A12 had significantly more radioactivity associated with PSMA + tumors than PSMA − tumors at 1h ( p = 0.002), 4h ( p = 0.004) and 24h ( p = 0.003). Contrarily, when comparing the radioactivity retention at 1 h, 4 h and 24 h for animals receiving [ 89 Zr]Zr-A11, the retention of radioactivity in PSMA + and PSMA − tumors was non-significant at 1 h ( p = 0.6), 4 h ( p = 0.8) and 24 h ( p = 0.3).

Article Snippet: The PSMA-positive cell line LNCaP (CRL-1740) and PSMA-negative cell line PC-3 (CRL-1435) were procured from ATCC (Manassas, VA, USA).

Techniques: Injection, Radioactivity